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Processing of ribosomal RNA precursor in isolated insect nuclei

✍ Scribed by M. Hughes; F.C. Kafatos


Book ID
118851996
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
369 KB
Volume
39
Category
Article
ISSN
0006-291X

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Isolation of ribosomal RNA precursors fr
✍ James M. Hill πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 422 KB

A method is presented for the isolation of ribosomal RNA precursors from lung. The very labile precursors of ribosomal RNA in mouse lungs can be isolated and preserved when lung tissue (i) is frozen in liquid nitrogen and pulverized, (ii) is homogenized in hypotonic buffer, (iii) nuclei are rapidly