A Chinese hamster ovary (CHO) cell line expressing recornbinant human interferon-y (IFN-y) was grown under glucose limitation in a chemostat at a constant dilution rate of 0.015 h-' with glucose feed concentrations of 2.75 mM and 4 2 5 mM. The changes in cell concentration that accompanied changes i
Process monitoring of the production of γ-interferon in recombinant chinese hamster ovary cells
✍ Scribed by Timothy K. Nadler; Sandeep K. Paliwal; Fred E. Regnier; Rahul Singhvi; Daniel I.C. Wang
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 410 KB
- Volume
- 659
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Asparagine linked (N-linked) glycosylation is an important modification of recombinant proteins, because the attached oligosaccharide chains can significantly alter protein properties. Potential glycosylation sites are not always occupied with oligosaccharide, and site occupancy can change with the
Since sialic acid content is known to be a critical determinant of the biological properties of glycoproteins, it is essential to characterize and monitor sialylation patterns of recombinant glycoproteins intended for therapeutic use. This study reports site-and branchspecific differences in sialyla