𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Problems in the radiometric assessment of protein and nucleic acid synthesis

✍ Scribed by K.G. Oldham

Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
762 KB
Volume
44
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Protein and nucleic acid synthesis is usually determined by measuring the incorporation of labeled precursors of low molecular weight. This is done either by autoradiographic methods or by measuring incorporation of the acid-soluble precursors into acid-insoluble products. Spurious incorporation of activity into these macromolecular products can result in three ways:

  1. By incorporation of impurities initially present in the precursor. 2. By degradation of the precursor to yield products that are incorporated into other macromolecules. 3. By incorporation of activity from t,he precursor into impurities in the macromolecular acceptor.

📜 SIMILAR VOLUMES

Contributions of mass spectrometry in th
Contributions of mass spectrometry in the study of nucleic acid-binding proteins and of nucleic acid–protein interactions
✍ Filippo Rusconi; François Guillonneau; Danièle Praseuth 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 628 KB

## Abstract | I. | Introduction | 306 | | II. | Supramolecular Structure Characterization | 308 | | | A. A Protein Is Used as a “Hook” | 309 | | |    1. Immuno‐Precipitation | 309 | | |    2. Tagging with a Poly‐Histidine Sequence | 310 | | |    3. Direct Analysis of Large Protein Complexes | 3