𝔖 Bobbio Scriptorium
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Problems encountered in the production of antibody to kinins and development of a radioimmunoassay

✍ Scribed by M.E. Webster; J.V. Pierce; M.U. Sampaio

Book ID
117196359
Publisher
Elsevier Science
Year
1969
Weight
220 KB
Volume
1
Category
Article
ISSN
0031-6989

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Rubratoxin B was coupled to ovalbumin using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide HC1 (ECDI) in high concentration at pH 8.0. Under these conditions it was possible to couple 13 moles of rubratoxin B per mole of ovalbumin. The conjugate was used for immunization of rabbits, and anti-rubratox