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Probing subtle acid-induced conformational changes of ribonuclease A by electrospray mass spectrometry

✍ Scribed by X.M Pan; X.R Sheng; S.M Yang; J.M Zhou


Book ID
117107001
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
370 KB
Volume
402
Category
Article
ISSN
0014-5793

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The thermal stability of ribonuclease S (RNase S), an enzymatically active noncovalent complex composed of a 2166-u peptide (S-peptide) and a 11,534-u protein (S-protein), was investigated by electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis ESI-MS (CE-ESI-MS). The int