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Probing protein stabilization by glycerol using electrospray mass spectrometry

✍ Scribed by Rita Grandori; Irena Matecko; Petra Mayr; Norbert Müller

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
115 KB
Volume
36
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

This study shows that electrospray ionization mass spectrometry (ESI‐MS), combined with a heated turbo ion‐spray interface, allows monitoring protein stabilization by glycerol in solution. Measurements obtained with the two proteins lysozyme and cytochrome c are presented. The observed mass‐to‐charge (m/z) distributions reveal the stabilizing effect of the additive on the protein conformations against temperature and acid‐induced unfolding, as well as against denaturation by acetonitrile. The data obtained with lysozyme allow detection of minor conformational changes upon glycerol addition to the native protein, and suggest that the protein structure in the presence of the additive is slightly compressed compared with its state in water. This result corroborates previous evidence obtained by nuclear magnetic resonance. It is also shown that analysis of the m/z distributions obtained by ESI‐MS can lead to detection of partially folded and partially populated states in protein samples. Copyright © 2001 John Wiley & Sons, Ltd.

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