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Probe diffusion in solutions of filamentous actin formed in the presence of gelsolin

✍ Scribed by Jay Newman; Gerlinde Gukelberger; Kenneth L. Schick; Ken S. Zaner

Publisher
Wiley (John Wiley & Sons)
Year
1991
Tongue
English
Weight
643 KB
Volume
31
Category
Article
ISSN
0006-3525

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✦ Synopsis

Dynamic light scattering was used to characterize the diffusion of monodisperse polystyrene latex spheres ( P L S ) of different sizes (55-, 105-, and 265-nm radii) in column-purified 0.65 mg/mL actin solutions polymerized with 100 m M KCl in the absence and presence of various amounts of the actin-binding protein gelsolin. The gelsolin and its interaction with actin was initially studied to ensure that the gelsolin could be used to produce filament populations with well-defined mean lengths. Measurements with PLS diffusion probes present showed, in the absence of gelsolin, that the effective local microviscosity in the actin solutions was 5-20 times that of water and that a large fraction of the PLS are trapped within the pores of the actin filament network, as found previously [

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