𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Primary structure of the tms and prs genes of Bacillus subtilis

✍ Scribed by Nilsson, Dan ;Hove-Jensen, Bjarne ;Arnvig, Kirsten

Publisher
Springer
Year
1989
Tongue
English
Weight
645 KB
Volume
218
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

The nucleotide sequence was determined of a 3211 nucleotide pair EcoRI-PvuII DNA fragment containing the tms and prs genes as well as a part of the ctc gene of Bacillus subtilis. The prs gene encodes phosphoribosylpyrophosphate (PRPP) synthetase, whereas the functioning of the tms and ctc gene products remains to be established. The prs gene contains an open reading frame of 317 codons resulting in a subunit Mr of 34828. An open reading frame comprising the tms gene contained 456 codons resulting in a putative translation product with an Mr of 49,554. Comparison of the deduced B. subtilis PRPP synthetase amino acid sequence with PRPP synthetases from Escherichia coli and rat liver showed extensive similarity. The deduced Tms amino acid sequence was found to be 43% similar to the deduced amino acid sequence of ecourfl, a gene of E. coli with unknown function.

πŸ“œ SIMILAR VOLUMES

On the identity of dnaP and dnaF genes o
On the identity of dnaP and dnaF genes of Bacillus subtilis
✍ Attolini, Carmen ;Mazza, Giorgio ;Fortunato, Adriana ;Ciarrocchi, Giovanni ;Mast πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 696 KB

The dnaP strains of Bacillus subtilis are altered in the initiation of DNA replication at high temperature (Riva et al., 1975). Fine mapping of the gene shows that it is located very close to the dnaF gene described by Karamata and Gross (1970) and mapped by Love et al. (1976) in the polC region. Th

Cloning and nucleotide sequence of the p
Cloning and nucleotide sequence of the ptsG gene of Bacillus subtilis
✍ Zagorec, M. ;Postma, P. W. πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 447 KB

The ptsG gene of Bacillus subtilis encodes Enzyme IIGlc of the phosphoenolpyruvate: glucose phosphotransferase system. The 3' end of the gene was previously cloned and the encoded polypeptide found to resemble the Enzymes IIIGlc of Escherichia coli and Salmonella typhimurium. We report here cloning

The regulation of the fumarase (citG) ge
The regulation of the fumarase (citG) gene of Bacillus subtilis 168
✍ Feavers, Ian M. ;Price, Vincent ;Moir, Anne πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 864 KB

The level of fumarase activity in Bacillus subtilis depends on the nutritional environment; in rich medium low vegetative levels increase towards the end of the exponential phase, whereas in minimal glucose medium levels are relatively high throughout growth. Analysis of the enzyme levels in spoO mu

Bacillus subtilis dnaF: A mutation of th
Bacillus subtilis dnaF: A mutation of the gene specifying the structure of DNA polymerase III
✍ Vrooman, Mary Jane ;Barnes, Marjorie H. ;Brown, Neal C. πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 495 KB

The characteristics of Bacillus subtilis dnaF, a mutation specifying a temperature sensitive phenotype, were examined to determine its relationship to polC, the gene specifying the structure of DNA polymerase III (pol III). Exposure of growing cells bearing dnaF to non-permissive temperature inhibit

Identification of the structural gene of
Identification of the structural gene of the PEP-phosphotransferase enzyme I in Bacillus subtilis marburg
✍ Niaudet, B. ;Gay, P. ;Dedonder, R. πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 711 KB

A thermosensitive mutation pt8I1 in the gene coding for the Enzyme I of the PEP-phosphotransferase system pathway has been isolated. The mutant enzyme was shown to be sensitive to high temperature, but this effect is dependent on the ionic strength. The ptsI1 mutation was shown to belong to the prev