Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist

✍ Scribed by Eisenberg, Stephen P.; Evans, Ron J.; Arend, William P.; Verderber, Evie; Brewer, Michael T; Hannum, Charles H.; Thompson, Robert C.

• Book ID: 109762520
• Publisher: Nature Publishing Group
• Year: 1990
• Tongue: English
• Weight: 826 KB
• Volume: 343
• Category: Article
• ISSN: 0028-0836
• DOI: 10.1038/343341a0

✦ Synopsis

Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-1 beta. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.