𝔖 Bobbio Scriptorium
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Primary antibody formation in vitro by mouse cells in a complete homologous system

✍ Scribed by P. G. Munder; M. Modolell; W. Raetz; G. A. Luckenbach

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
401 KB
Volume
3
Category
Article
ISSN
0014-2980

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✦ Synopsis

Abstract

Mouse lymphocytes have been cultivated in the absence of heterologous sera or mitogenic compounds. The fetal calf serum usually used was replaced by small amounts (0.5–1 mg protein/ml medium) of homologous exudate fluid induced by intraperitoneal injection of lysolecithin. Under these conditions, a primary immune response against various antigens (sheep red blood cells, dinitrophenyl‐dextran, dinitrophenyl‐human IgG) can be elicited. Adjuvant activity of lysolecithin has also been studied in this system.

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