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Antigen-antibody complexes suppress antibody production by mouse plasmacytoma cells in vitro

✍ Scribed by A. K. Abbas; G. G. B. Klaus

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
400 KB
Volume
8
Category
Article
ISSN
0014-2980

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✦ Synopsis

Abstract

Preformed complexes of 2,4‐dinitrophenylated keyhole limpet hemocyanin (DNP‐KLH) and anti‐DNP or anti‐KLH antibodies selectively, suppressed the production of anti‐DNP IgA by the plasmacytoma cell line, MOPC 315, in vitro. Complexes made with DNP~8.5~KLH (8.5 moles DNP/10^5^ daltons carrier) inhibited the secretion of IgA but not its synthesis, whereas complexes prepared with the more highly substituted DNP~20~KLH inhibited synthesis as well. Moreover, complexes of DNP‐KLH and anti‐DNP F(abβ€²)~2~ fragments were as suppressive as those made with IgG antibodies, suggesting that the mode of presentation of antigenic determinants and not surface Fc receptors may play a major role in this phenomenon.

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## Abstract Mouse lymphocytes have been cultivated in the absence of heterologous sera or mitogenic compounds. The fetal calf serum usually used was replaced by small amounts (0.5–1 mg protein/ml medium) of homologous exudate fluid induced by intraperitoneal injection of lysolecithin. Under these c