## Abstract The presence of both hepatitis B virus (HBV) DNA and HBV antigens (HBsAg, HBeAg) was assessed in peripheral blood mononuclear cells (PBMC) from 32 patients chronically infected with HBV. Three different molecular forms of HBV DNA were observed: free monomers (5), highβmolecularβweight f
Prevalence and significance of hepatitis B virus antigens: Expression in peripheral blood mononuclear cells in chronic active hepatitis
β Scribed by P. Parvaz; J.-P. Lamelin; L. Vitvitski; P. Bouffard; C. Pichoud; L. Cova; C. Trepo
- Book ID
- 118374856
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 547 KB
- Volume
- 43
- Category
- Article
- ISSN
- 1090-2341
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In this study, peripheral-blood mononuclear cells from patients with chronic hepatitis B and spontaneous or therapy-induced disappearance of HBsAg were examined for HBV DNA. Samples were evaluated by in situ hybridization and polymerase chain reaction both before and after clearance of HBsAg. By in
## Abstract Hepatitis B virus (HBV) infection of peripheral blood mononuclear cells (PBMCs) has been observed in all stages of liver disease. Thus far all information about the physical state of HBV in mononuclear blood cells comes from Southern blot analysis and in situ hybridization. In this stud
## Abstract Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglut