## Abstract Hepatitis B virus (HBV) infection of peripheral blood mononuclear cells (PBMCs) has been observed in all stages of liver disease. Thus far all information about the physical state of HBV in mononuclear blood cells comes from Southern blot analysis and in situ hybridization. In this stud
Hepatitis B Virus Antigens in Peripheral Blood Mononuclear Cells during the Course of Viral Infection
β Scribed by Felix R. Roisman; Alejandro Castello; Hugo Fainboim; Adrian Morelli; Leonardo Fainboim
- Book ID
- 115594094
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 409 KB
- Volume
- 70
- Category
- Article
- ISSN
- 1090-2341
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The hepatitis B virus genome encodes a transcriptional transactivator protein designated HBxAg. We have investigated whether this antigen is a target structure for human T-lymphocytes. Using recombinant HBxAg protein, we found HBxAg-specific stimulation of peripheral blood mononuclear cells in patie
## Abstract Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglut
## Abstract The presence of both hepatitis B virus (HBV) DNA and HBV antigens (HBsAg, HBeAg) was assessed in peripheral blood mononuclear cells (PBMC) from 32 patients chronically infected with HBV. Three different molecular forms of HBV DNA were observed: free monomers (5), highβmolecularβweight f