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Presynaptic inhibition of excitatory neurotransmission by lamotrigine in the rat amygdalar neurons

✍ Scribed by Su-Jane Wang; Chiung-Chun Huang; Kuei-Sen Hsu; Jing-Jane Tsai; Po-Wu Gean

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 687 KB
Volume: 24
Category: Article
ISSN: 0887-4476
DOI: 10.1002/(sici)1098-2396(199611)24:3<248::aid-syn7>3.0.co;2-e

No coin nor oath required. For personal study only.

✦ Synopsis

Lamotrigine (LAG)

is a new antiepileptic drug which is licensed as adjunctive therapy for partial and secondary generalized seizures. In the present study, the mechanisms responsible for its antiepileptic effect were studied in rat amygdaloid slices using intracellular recording and whole-cell patch clamp techniques. Bath application of LAG (50 pM) reversibly suppressed the excitatory postsynaptic potentials (EPSPs) and currents (EPSCs) evoked by stimulating ventral endopyriform nucleus. Synaptic response mediated by the N-methyl-D-aspartate (NMDA) receptor (EPSP,MDa) was isolated pharmacologically by application of a solution containing non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX,10 pM) and y-aminobutyric acidA receptor antagonist bicuculline (20 pM). LAG produced a parallel inhibition of EPSPNMDA. Postsynaptic depolarization induced by cu-amino-5-methyl-4-isoxazole propionate (AMPA) was not altered by LAG. In addition, LAG increased the ratio of the second pulse response to the first pulse response (Pz/pl), which is consistent with a presynaptic mode of action.

The L-type Ca++ channel blocker nifedipine (20 pM) had no effect on LAG-induced presynaptic inhibition. However, the depressant effect of LAG was markedly reduced in slices pretreated with N-type Ca' + channel blocker o-conotoxin-GVIA (o-CgTX-GVIA, 1 pM) or a broad spectrum Cat+ channel blocker o-conotoxin-MVIIC (o-CgTX-MVIIC, 1 pM). It is concluded that a reduction in w-CgTX-GVIA-sensitive Ca++ currents largely contributes to LAG-induced presynaptic inhibition.

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