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Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction

✍ Scribed by Kai Zhang; Ruyu Gao; Zhengjin Jiang; Chuanyi Yao; Zhichao Zhang; Qinsun Wang; Chao Yan

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
513 KB
Volume
26
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A pressurized capillary electrochromatography (pCEC) instrument with solvent gradient capability has been used for the separation of a peptide mixture. Retention mechanism and selectivity of the peptides were studied by pCEC using a strong cation exchange (SCX) column. The effects of applied voltage, supplementary pressure, organic modifier concentration, ionic strength, and pH value on pCEC separation were investigated. It was found that the retention mechanism of the peptides in this system is based on a mixed mode of hydrophilic interaction, strong cation exchange, and electrophoresis. Compared with the separation results obtained by reverse phase pCEC and capillary electrophoresis (CE), this mixed‐mode pCEC is more powerful for the separation of hydrophilic peptides with similar charge‐to‐mass ratio.

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