Two-dimensional strong cation-exchange liquid chromatography/reversed-phase pressurized capillary electrochromatography for separation of complex samples

✍ Scribed by Yi Wu; Yan Wang; Xue Gu; Lin Zhang; Chao Yan

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 337 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000815

✦ Synopsis

Abstract

A 2‐D separation platform was constructed using micro strong cation‐exchange liquid chromatography (μ‐SCXLC) and reversed‐phase pressurized capillary electrochromatography (RP‐pCEC) for the analysis of complex samples. Samples were fractionated by the first‐dimension μ‐SCXLC with a linear solvent gradient and then injected into the second‐dimension RP‐pCEC for further separation. The μ‐SCXLC/RP‐pCEC 2‐D system with three separation mechanisms, namely strong cation‐exchange, reversed‐phase chromatography and electrophoresis, provided high selectivity, high resolution and high peak capacity compared to one‐dimensional chromatographic approaches. Separation effectiveness of this 2‐D system was demonstrated by the analysis of different kinds of complex samples, such as traditional Chinese medicine Cortex Phellodendri, bovine serum albumin (BSA) tryptic digest and real serum tryptic digest. A theoretical peak capacity of approximately 1200 was achieved, which proves its promising potential for the separation and analysis of complex samples.