## Abstract The temporal relation between hormone uptake, metabolism, and reinitiation of meiosis was investigated following in vitro incubation of immature starfish oocytes with the maturation inducing hormone 1‐methyladenine (1‐MA) and/or ^3^H‐1‐methyladenine (^3^H‐1‐MA). Oocyte incorporation of
Pressure inhibition of starfish oocyte maturation: Temporal aspects
✍ Scribed by Cloud, J. G.
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 572 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
High hydrostatic pressure reversibly inhibits l-methyladenine (1-MA)-induced maturation of starfish oocytes; the objective of this investigation was to describe more fully the nature of this inhibition. Oocytes incubated at atmospheric pressure following a 90-min period with 1-MA at
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## Abstract Fully grown oocytes of starfish __(Asterias forbesi)__ are normally arrested in the dictyate stage of meiosis within the ovary. Reinitiation of meiotic maturation in these oocytes appears to be induced as a result of the interaction of 1‐methyladenine (1‐MA) with the oocyte surface. The
Maturation-promoting factor (MPF) has been known to be produced in the cytoplasm of starfish and amphibian oocytes, where it triggers meiotic maturation. Cytoplasm obtained from maturing oocytes of either mouse or surf clam was found to induce maturation when injected into immature starfish oocytes.
Cross-reactivity of maturation-inducing factors was studied between sea cucumbers and starfish. Radial nerve extracts from both sea cucumbers and starfish induce maturation in ovarian oocytes associated with follicle cells but not in follicle-free oocytes. Radial nerve extracts of sea cucumbers did
## Abstract A factor that blocks serotonin (5‐hydroxytryptamine, 5‐HT)‐induced maturation of __Spisula__ oocytes was purified from __Spisula__ oocytes by ultrafiltration through a PM‐10 membrane, charcoal adsorption, and reversed‐phase HPLC on a Partisil ODS‐3 column, The oocyte factor is a heat‐st