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Preparative separation of human B and T lymphocytes by free flow electrophoresis

✍ Scribed by T.G. Baier; G. Weber; K. Hartmann; U. Heinrich; D. Schönberg

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
398 KB
Volume
171
Category
Article
ISSN
0003-2697

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✦ Synopsis

An electrophoretic method for the quantitative separation of human B and T lymphocytes in a carrier-free system is presented. The method is based on the fact that B and T lymphocytes show marked overlap in their size and density characteristics, but differ sufficiently in surface charge to be separable by electrophoresis. The technique is performed in phosphate-buffered saline and appears to be especially suitable for the enrichment of nonstimulated, functionally intact lymphocytes which can be directly used for further immunological or biochemical studies.

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