Preparative separation of ganglioside GM3 by high-performance liquid chromatography
β Scribed by R.F. Menzeleev; Yu.M. Krasnopolsky; E.N. Zvonkova; V.I. Shvets
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 360 KB
- Volume
- 678
- Category
- Article
- ISSN
- 1873-3778
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Analytical and preparative procedures are described for high-performance liquid chromatography (HPLC) fractionation of gangliosides without previous derivatization. These procedures make use of a reversed-phase Lichrosorb RB-8 or pBondapak RP-18 column, and of a mixture of acetonitrile and 5 niM pho
## Abstract A method is presented for separation of tryptic glycopeptidesβcontaining oligosaccharides of the Nβasparagineβlinked type. High performance liquid Chromatography (HPLC) of glycopeptides on a C18 reverseβphase system eluted with a gradient of 0%β50% acetonitrile in 0.1 M NaPO~4~ pH 2.2 r
## Copyright 'i, lY79 hy Academtc Prcr\. Inc A,, right\ ill rcyroductmn ,n any t,rm rcrrncd. ' Similar problems with underivatired silica are presented in Ref. (10).