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Preparative-scale isolation of isotopically labeled amino acids

✍ Scribed by David M. LeMaster; Frederic M. Richards

Book ID: 102984639
Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 847 KB
Volume: 122
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90275-5

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✦ Synopsis

Over 10 g of individual ZH,'5N-labeled amino acids was resolved and recovered on a laboratory-scale ion-exchange system from a crude bacterial protein hydrolyzate derived from 20 g of lyophilized cells. The 17 amino acids (cystine was not isolated) were recovered containing less than 1.0% of other contaminating amino acids except for proline (4.0%). The aromatic and basic amino acids were isolated on a dual-column carrier displacement system (390-ml resin bed volume) while most of the neutral and acidic amino acids were separated on a pyrazolium chloride elution system (560-ml resin bed volume). The two remaining overlapping pairs were resolved on small carrier displacement columns. In addition, the overlapping fractions from adjacent peaks of the pyrazolium chloride elution system represent only 3.5% (0.37 g) of the total sample.

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