The recovery of proteins in polyacrylamide gel electrophoresis (PAGE) has not been previously investigated systematically. The fractionation of hemoglobins A and 8 (1) by which milligram-preparative PAGE (2) was initially introduced yielded nearly quantitative recovery of hemoglobin, a finding which
Preparative polyacrylamide gel electrophoresis
โ Scribed by Piergiorgio Righetti; Camillo Secchi
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 831 KB
- Volume
- 72
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
The construction and operation of a preparative polyacrylamide-gel electrophoresis system is described. Emerging protein bands are collected by an intermittent pumping system which is based on the design of Brownstone ((1%9) Anal. Biochem. 27, 25-46). The original pressure-sensitive operation was,
## A method that is both simple and inexpensive is described for preparative polyacrylamide gel electrophoresis of low-molecular-weight RNA. The utility of the method is demonstrated by the preparative separation of 4S and 5s RX-i.
The Shandon Southern preparative polyacrylamide gel electrophoresis apparatus was modified to permit use with SDS (sodium dodecyl sulfate) containing systems. Improvements include the elimination of the troublesome lower gel and modification of the elution plate to prevent localized high current den