## Recent developments in preparative free flow isoelectric focusing Continuous flow electrophoresis (CFE) is a promising method for preparative fractionation of a variety of biological species, ranging from peptides and proteins to subcellular particles and cells. The high separation efficiency o
Preparative free-flow isoelectric focusing: Modeling and experiments
✍ Scribed by Dr. Martine Poux; Joël Bertrand
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 585 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0173-0835
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## Abstract Continuous‐flow isoelectric focusing (IEF) has the potential to be an important method in proteome analysis. The current devices do not fully use the advantages of IEF, because they do not utilize all its important features including changes in background conductivity during the focusin
A multi-chamber apparatus for preparative isoelectric focusing is described. The apparatus is constructed of 32 separation chambers and 2 electrode chambers, all separated by uncharged porous membranes. The total volume of the 32 separation chambers is 660 mL. A cooling system and a stirring system
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