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Multi-chamber apparatus for preparative isoelectric focusing

✍ Scribed by Dr. Jan Kyhse-Andersen

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
547 KB
Volume
10
Category
Article
ISSN
0173-0835

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✦ Synopsis

A multi-chamber apparatus for preparative isoelectric focusing is described. The apparatus is constructed of 32 separation chambers and 2 electrode chambers, all separated by uncharged porous membranes. The total volume of the 32 separation chambers is 660 mL. A cooling system and a stirring system are built in. Human serum proteins were separated by isoelectric focusing in a natural pH gradient. The fractionation was monitored by fused rocket immunoelectrophoresis. The number of proteins in each fraction was monitored by crossed inimunoelectrophoresis. The apparent p l values of IgG, transferrin and alpha-1-antitrypsin are as found in the literature. Orosomucoid (alpha-1 -acid glycoprotein) (PI = 1.8) is concentrated at the acid end of the pH gradient.

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