Preparative capillary zone electrophoresis using a dynamic coated wide-bore capillary
β Scribed by Mahmoud M. Yassine; Charles A. Lucy
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 294 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0173-0835
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π SIMILAR VOLUMES
CZE allows to measure the acidic dissociation constant (pKa) of many drug substances. However, determining the EOF intensity may be time-consuming, especially at a low pH. In order to overcome this drawback, a dynamic coating procedure of the capillary was carried out to increase microEOF, and thus
## Abstract This paper describes a simple means of obtaining high resolution separations of basic proteins at a pH below their pl. Small amounts of a cationic fluorosurfactant are added to the running buffer. A positively charged wall is thereby obtained which will repel positively charged proteins