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A new approach to dynamic deactivation in capillary zone electrophoresis

✍ Scribed by Emmer, Åsa ;Jansson, Mårten ;Roeraade, Johan

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
243 KB
Volume
14
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

This paper describes a simple means of obtaining high resolution separations of basic proteins at a pH below their pl. Small amounts of a cationic fluorosurfactant are added to the running buffer. A positively charged wall is thereby obtained which will repel positively charged proteins. The particular chemistry of fluorosurfactants is believed to enhance the efficiency of the deactivation. Examples are presented of the separation of some model proteins, including a human insulin‐like growth factor (IGF‐I), and a misfolded by‐product of the growth factor.

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A simple means to increase absorbance de
A simple means to increase absorbance detection sensitivity in capillary zone electrophoresis
✍ Shaorong Liu; Purnendu K. Dasgupta 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 630 KB

Up to a threefold gain in sensitivity without loss of separation efficiency can be achieved by the simple expedient of connecting a larger diameter capillary at the measurement point. This is unique to electroosmotically pumped systems. Theoretical considerations are outlined and experimental verifi