✦ LIBER ✦

A simple means to increase absorbance detection sensitivity in capillary zone electrophoresis

✍ Scribed by Shaorong Liu; Purnendu K. Dasgupta

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 630 KB
Volume: 283
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)85289-v

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✦ Synopsis

Up to a threefold gain in sensitivity without loss of separation efficiency can be achieved by the simple expedient of connecting a larger diameter capillary at the measurement point. This is unique to electroosmotically pumped systems. Theoretical considerations are outlined and experimental verification is presented. With measurement performed by presently available commercial absorbance detectors designed for on-column detection, the gain in detectabiities can be very substantial when capillaries smaller than 75 pm in bore are used for separation.

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