Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis

✍ Scribed by Toru Takano; Takuya Higashiyama; Takashi Uruno; Hiroya Yamada; Hiroshi Yoshida; Akira Miyauchi

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 257 KB
• Volume: 30
• Category: Article
• ISSN: 1043-3074
• DOI: 10.1002/hed.20810

✦ Synopsis

Abstract

Background

The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin‐3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult.

Methods

We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber‐EP4 antibody and the other was filtration through a nylon mesh.

Results

After selection with Ber‐EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases.

Conclusion

Mesh filtration of aspirates proved superior results for the measurement of T/G ratio. © 2008 Wiley Periodicals, Inc. Head Neck, 2008