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Preparation of the Phosphatase Reagent Disodium p-Nitrophenyl Phosphate

โœ Scribed by ASCHAFFENBURG, R.

Book ID
121145904
Publisher
American Association for the Advancement of Science
Year
1953
Tongue
English
Weight
65 KB
Volume
117
Category
Article
ISSN
0036-8075

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The phosphorylase phosphatase activity of the ATP,Mgdependent protein phosphatase is stimulated by p-nitrophenyl phosphate (pNPP). All the active forms of this type of enzyme show this property, which seems to be unrelated to anypNPP-hydrolyzing activity. The increase in activity is due to an increa