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Stimulation of the ATP, Mg-dependent protein phosphatase by p-nitrophenyl phosphate

✍ Scribed by Jozef Goris; Wilfried Merlevede

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 478 KB
Volume: 171
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90509-x

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✦ Synopsis

The phosphorylase phosphatase activity of the ATP,Mgdependent protein phosphatase is stimulated by p-nitrophenyl phosphate (pNPP). All the active forms of this type of enzyme show this property, which seems to be unrelated to anypNPP-hydrolyzing activity. The increase in activity is due to an increase in V,,,, the Km being unchanged. The possibility that pNPP acts as a deinhibitor is excluded. pNPP acts as a competitive inhibitor on the phosphorylase phosphatase activity of the different polycation-stimulated protein phosphatases. Stimulation by pNPP can be used as a differential criterion in a specific assay of the active forms of the ATP,Mg-dependent phosphatase. o 1988 Academic press, II-K.

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