𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparation of single-celled marine dinoflagellates for electron microscopy

✍ Scribed by Truby, Earnest W.

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
172 KB
Volume
36
Category
Article
ISSN
1059-910X

No coin nor oath required. For personal study only.

✦ Synopsis

Electron microscopy has been used successfully to study and identify single-celled marine dinoflagellates including parasitic ones and others, such as those that cause red tide. Delicate cells can be preserved for scanning electron microscopy with a combined glutaraldehydeosmium tetroxide mixture that is adjusted for the osmolality of the medium. The protocol allows resolution of fine morphological features. Preservation for transmission electron microscopy can be accomplished with a standard glutaraldehyde fixation and osmium-tetroxide post-fixation in a suitable buffer, but again, the osmolality of the mixture must be adjusted. The protocol allows ultrastructural resolution of vesiculated cells and has been modified for small sample sizes.

📜 SIMILAR VOLUMES

Construction of a cooling and heating su
Construction of a cooling and heating substrate holder for the preparation of thin films for electron microscopy
✍ Ott, Philipp; G�nter, John Ralph 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB

The PVD preparation of thin, oriented films for electron microscopy of materials with high adatom mobilities on the substrates requires cooling of the substrate holder. The construction of such a holder for cooling the substrate to -50 and heating it to 500 degrees C is described.

A rapid method for the preparation of si
A rapid method for the preparation of single-cell suspensions from solid tissues
✍ Narendra P. Singh 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 66 KB 👁 2 views

A simple and rapid methodology for the preparation of single-cell suspension from animal tissues is described. It uses a device called a "tissue press" to mince the tissue into small pieces of desired sizes which are then dissociated into single cells using a pipette. The device is inexpensive and t

Rücktitelbild: Coupling Amperometry and
Rücktitelbild: Coupling Amperometry and Total Internal Reflection Fluorescence Microscopy at ITO Surfaces for Monitoring Exocytosis of Single Vesicles (Angew. Chem. 22/2011)
✍ Anne Meunier; Ouardane Jouannot; Dr. Rémy Fulcrand; Dr. Isabelle Fanget; Dr. Mar 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 431 KB

Exozytose … … ist ein Schlüsselmechanismus, durch den ein zellulärer Organismus molekulare Botenstoffe in das extrazelluläre Medium freisetzt. C. Amatore et al. berichten in ihrer Zuschrift auf S. 5187 ff. über die Entwicklung eines ITO-Mikroinstruments, das zwei leistungsfähige Techniken -Amperomet