The expression pattern, enzymatic activity, and products of 8-lipoxygenase (LOX) were analyzed in normal and neoplastic skin of NMRI mice. While barely detectable in normal epidermis, 8-LOX was transiently induced by 12-O-tetradecanoylphorbol-13-acetate and constitutively expressed in papillomas but
Preparation of oxygen-18-labeled lipoxygenase metabolites of arachidonic acid
β Scribed by Jay Y. Westcott; Keith L. Clay; Robert C. Murphy
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 476 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1076-5174
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Stereospecifically 3 H-labeled substrates are useful tools in studying the mechanism of hydrogen abstractions involved in the oxygenation of polyunsaturated fatty acids. Here, we describe modified methods for the synthesis of arachidonic acids labeled with a single chiral tritium on the methylene gr
## Abstract Methods are presented for the preparation on 10^β3^ mole scale of nitromethane and methyl nitrate enriched with oxygenβ18 or nitrogenβ15. Enrichment in each atomic position is discussed.
Oxygen-18 isotope labeled at the carboxyl group of glycine, L-alanine and L-proline were synthesized by acid catalyzed exchange or acid hydroll1;s of respective methyl ester derivatives of amino acids in HCl/H . Quantitative enrichment of ycine was achieved by the acid hydrofysis of amino fietonitri