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Preparation of ligation intermediates and related polynucleotide pyrophosphates

โœ Scribed by Barbara C.F. Chu; Leslie E. Orgel

Book ID
119119326
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
242 KB
Volume
782
Category
Article
ISSN
0167-4781

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Nuclear ligation of RNA polynucleotide k
Nuclear ligation of RNA polynucleotide kinase products
โœ Ilga Winicov ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 1005 KB

## Abstract RNA polynucleotide kinase has been shown to transfer [ฮณ^32^P] from ATP to 5โ€OH termini of endogenous nuclear RNA. The products of this reaction have been isolated in RNA larger than 125 after in vitro incubation of mouse L cell nuclei. About 20%โ€“30% of these 5โ€ฒโ€OH kinase products are po