## Abstract RNA polynucleotide kinase has been shown to transfer [Ξ³^32^P] from ATP to 5βOH termini of endogenous nuclear RNA. The products of this reaction have been isolated in RNA larger than 125 after in vitro incubation of mouse L cell nuclei. About 20%β30% of these 5β²βOH kinase products are po
The preparation and stability of immobilized polynucleotide kinase
β Scribed by D. W. Hutchinson; R. Collier
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 263 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0006-3592
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## Abstract The effect of ribose(O2β²)βmethylation on the stability of (O2β²)βmethylated polyribonucleotide helices has been studied by conformational energy calculations. The preferred orientation of the methyl group is found to further stabilize the helical phosphodiester conformation (__g__^β^,__g
Mammalian polynucleotide kinases (PNKs) carry out 5Π-phosphorylation of nucleic acids. Although the cellular function(s) of these enzymes remain to be delineated, important suggestions have included a role in DNA repair and, more recently, in DNA replication. Like T4 PNK, some preparations of mammal