Preparation of immobilized swainsonine analogs on solid support

✍ Scribed by William H Pearson; Luyi Guo; Tanya M Jewell

• Publisher: Elsevier Science
• Year: 2002
• Tongue: French
• Weight: 167 KB
• Volume: 43
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(02)00254-x

✦ Synopsis

Golgi a-mannosidase II and lysosomal mannosidase are examples of biologically important a-mannosidases that are difficult to purify. Swainsonine is an effective inhibitor of these a-mannosidases. In this report, analogs of swainsonine bearing an aminoalkyl group at C3 or C6 are synthesized and coupled with Affi-Gel 10 to prepare affinity matrices that may be useful for the preparation of a-mannosidases. Model ligands, where the aminoalkyl group is capped with an acyl group, are evaluated against a commercial a-mannosidase.