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Preparation of dipteran larvae for scanning electron microscopy using a freeze-substitution technique

✍ Scribed by Colwell, Douglas D.; Kokko, Eric G.

Book ID
125843537
Publisher
NRC Research Press
Year
1986
Tongue
English
Weight
263 KB
Volume
64
Category
Article
ISSN
0008-4301

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A combined technique of the rapid freezing, freeze substitution-fixation method and the osmium-DMSO-osmium method was devised. By this combined method we clearly observed the architecture of intracellular components in three dimensions. Morphological characteristics were generally similar to those o