Freeze-substitution techniques for preparing nematodes for scanning electron microscopy
β Scribed by D. A. Wharton
- Book ID
- 119836229
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 872 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0022-2720
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A combined technique of the rapid freezing, freeze substitution-fixation method and the osmium-DMSO-osmium method was devised. By this combined method we clearly observed the architecture of intracellular components in three dimensions. Morphological characteristics were generally similar to those o