Preparation of deuterium labelled catecholamines, catecholamine precursors and metabolites for use as internal standards in mass fragmentographic determination and for turnover studies

✍ Scribed by B. Lindström; B. Sjöquist; E. Anggard

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: French
• Weight: 255 KB
• Volume: 10
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2590100202

✦ Synopsis

L-dihydroxyphenytalanine-H3 L-tyrwsine-2HZ dopamine-H 3methoxytyramine-H3

' H 5 and vanillylmzndetic acid H2 were prepared by hydrogen-deuterium exchange under acidic o r basic conditions. 4-hydroxy-3methoxy phenylethyleneglycol-H3 was prepared f m m vanillylman-2 2 d e t i c acid-H by reduction with LiAl H4. The isotopic y i e l d f o r 6-dihydroxyphenylalanine was 90 X and f o r the other compounds >99 %. These labelled compounds could be used as internal standards i n the mass fragmentographic analysis of the protiwn species i n tissues and body f l u i d s as well as i n metabolic studies vivo.

2 homovanit l i e acid-2Hhomovanillic acid--

Recently mass spectrometric techniques have become available for the specific determination of endogenous and exogenous compounds with high degree of precision and . These methods require the availability of the isotopically labelled speaies ak an internal standard. For many purposes incorporations of deuterium by simple exchange or reduction reactions provide compounds with the desired properties.

Most of our knowledge of the catecholamines in the brain has been obtained in experimental animals where it is possible to ob'tain