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Preparation of avidin-labeled protein nanoparticles as carriers for biotinylated peptide nucleic acid

✍ Scribed by Klaus Langer; Conrad Coester; Carolin Weber; Hagen von Briesen; Jörg Kreuter

Book ID
117639104
Publisher
Elsevier Science
Year
2000
Tongue
French
Weight
308 KB
Volume
49
Category
Article
ISSN
0939-6411

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✍ V.V. Didenko 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 389 KB

We have used M13 single-stranded DNA bound by uv to small pieces of nylon membrane for the synthesis of biotinylated single-stranded DNA probes. The labeling method requires a large fragment of DNA polymerase I and random hexanucleotides. There is no need for previous linearization of the template.