Preparation of 14C-fluorescein isothiocyanate
✍ Scribed by E.A. Johnson; W.D. Brighton
- Book ID
- 102625733
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 259 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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## Abstract A fast and convenient microwave assisted one‐pot synthesis of methyl‐[^14^C]‐isothiocyanate 4 was shown. The continued one‐pot synthesis with 4 to a highly refined material like [5‐^14^C]‐dimethylsulfanyltriazolepyridines 8 and 13 without any intermediate purification, six steps in the
The method is fast and simple, and the amount of material fractionated can be scaled up or down as desired without appreciable loss of resolution. Amounts ranging from 10 mg up to 2 gm of arginine-rich histones were successfully fractionated on columns proportional to the chromatographed sample.