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Preparation of 125I-labeled secretin of high specific radioactivity

✍ Scribed by Hsin-Hsiung Tai; Brian Korsch; William Y. Chey


Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
501 KB
Volume
69
Category
Article
ISSN
0003-2697

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✦ Synopsis


A simple and rapid method of preparing 1251-labeled secretin of high specific radioactivity has been developed. Synthetic porcine secretin was iodinated with Na[rz51] by a modification of the chloramine T method. Purification and separation of labeled from unlabeled secretin was achieved by chromatography on SP-Sephadex C-25 column. The labeled secretin possessed specific radioactivity as high as 500-550 &i/pg. 34


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