Amiloride and certain of its derivatives are effective inhibitors of Na/H antiporters and of epithelial Na channels. We describe a simple method for the preparation of a variety of pharmacologically active 6-iodoamiloride derivatives that are labeled with 125I at high specific radioactivity. 6-Dechl
Preparation and purification of 125I-labeled iodospiroperidol
โ Scribed by Scott W. Landvatter
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- French
- Weight
- 187 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0022-2135
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โฆ Synopsis
Iodine-125 labeled spiroperidol has been synthesized by a simple, one-step procedure starting from spiroperidol. and H202 in buffered acetic acid. reaction affords iodospiroperidol in 40% radiochemical yield and a specific activity, after HPLC purification, of ca. 1900 Ci/mmol.
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A simple and rapid method of preparing 1251-labeled secretin of high specific radioactivity has been developed. Synthetic porcine secretin was iodinated with Na[rz51] by a modification of the chloramine T method. Purification and separation of labeled from unlabeled secretin was achieved by chromato