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Preparation, isolation, and characterization of novel heterogeneous branched cyclomalto-oligosaccharides having β-d-galactosyl residue(s) on the side chain

✍ Scribed by Kyoko Koizumi; Toshiko Tanimoto; Koki Fujita; Koji Hara; Nobuhiro Kuwahara; Sumio Kitahata

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 912 KB
Volume: 238
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(93)87006-e

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✦ Synopsis

Transgalactosylated products of branched cyclodextrins (glucosyl-alpha CD, -beta CD, -gamma CD, and maltosyl-alpha CD, -beta CD, -gamma CD) were synthesized by beta-D-galactosidases from Bacillus circulans and Penicillium multicolor using lactose as a donor substrate and branched CDs as acceptors. Eighteen beta-D-galactosylated branched CDs were isolated and purified by HPLC. Their structures were elucidated by FABMS and 13C NMR spectroscopies, and methylation analysis. The chromatographic behavior of these novel heterogeneous branched CDs on three HPLC columns of different separation modes was compared.

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