Isolation by HPLC of the positional isomers of heterogeneous doubly branched cyclomaltohexaose having one α-d-galactosyl and one α-d-glucosyl side chain and determination of their structures by enzymatic degradation
✍ Scribed by Yasuyo Okada; Kyoko Koizumi; Sumio Kitahata
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 523 KB
- Volume
- 287
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Each of the six and five positional isomers of 2n- and 6n-O-alpha-D-galactopyranosyl-6(1)-O-alpha-D-glucopyranosyl-cyclomalt ohexaose (alpha-cyclodextrin, alpha CD; 2n: n = 1-6, 6n: n = 2-6), which were produced from 6-O-alpha-D-glucopyranosyl-alpha CD and melibiose by transgalactosylation with coffee bean alpha-galactosidase, were isolated by high-performance liquid chromatography (HPLC) on a reversed-phase column and a graphitized carbon column. The structures of those isomers were elucidated by analyses of enzymatic degradation products with cyclomaltodextrin glucanotransferase and glucoamylase.