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Preparation and properties of thermoreversible, phase-separating enzyme-oligo(N-isopropylacrylamide) conjugates

✍ Scribed by Chen, Guohua; Hoffman, Allan S.


Book ID
126790600
Publisher
American Chemical Society
Year
1993
Tongue
English
Weight
796 KB
Volume
4
Category
Article
ISSN
1043-1802

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Unusual properties of thermally sensitiv
✍ Ding, Zhongli ;Chen, Guohua ;Hoffman, Allan S. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 164 KB

Reversible soluble-insoluble oligomer-enzyme conjugates have been prepared by conjugating a thermally sensitive oligomer, poly(N-isopropylacrylamide) [poly-(NIPAAm)] to trypsin. The conjugates can catalyze enzymatic reactions in solution and then may be separated from the solution by thermal precipi

Preparation of a thermally phase-separat
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## Abstract __N__‐isopropylacrylamide and __N__‐acryloxysuccinimide have been copolymerized in various mixtures of terrahydrofuran and toluene using azobisisobutyronitrile as initiator. Polymerization has been conducted for 24 h at 50Β°C under a slightly positive pressure of nitrogen. The copolymers