Preparation and properties of thermoreversible, phase-separating enzyme-oligo(N-isopropylacrylamide) conjugates
β Scribed by Chen, Guohua; Hoffman, Allan S.
- Book ID
- 126790600
- Publisher
- American Chemical Society
- Year
- 1993
- Tongue
- English
- Weight
- 796 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1043-1802
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π SIMILAR VOLUMES
Reversible soluble-insoluble oligomer-enzyme conjugates have been prepared by conjugating a thermally sensitive oligomer, poly(N-isopropylacrylamide) [poly-(NIPAAm)] to trypsin. The conjugates can catalyze enzymatic reactions in solution and then may be separated from the solution by thermal precipi
## Abstract __N__βisopropylacrylamide and __N__βacryloxysuccinimide have been copolymerized in various mixtures of terrahydrofuran and toluene using azobisisobutyronitrile as initiator. Polymerization has been conducted for 24 h at 50Β°C under a slightly positive pressure of nitrogen. The copolymers