Preparation and properties of phosphatidal ethanolamine from bovine white matter

✍ Scribed by M.F. Frosolono; M. Marsh

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 538 KB
• Volume: 10
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(73)90001-7

✦ Synopsis

The preparation of phosphatidal ethanolamine (Pal-E) from the ethanolamine phosphatide (EP) fraction of bovine brain white matter is described. The method is based upon the resistance of the plasmalogen 2-acyl linkage to mild alkaline hydrolysis in the presence of methanol and in the absence of chloroform. The average yield was 62% of the Pal-E originally present in the EP preparations. The IR, NMR, and ORD spectra of Pal-E were as expected on the basis of the groups present. The average molar absorbancy index at 6.02 ~t was 177. The presence of signals at 260.5 and 254.5 cpm in the NMR spectrum, along with the results obtained from the IR spectrum, allowed the unequivocal assignment of the cis-configuration to the 1-alkenyl linkage. No deviations from plain positive ORD curves were seen. The distribution of hydrocarbon residues was ascertained from GLC. The aldehydogenic residues on the 1-position contained 41% of normal olefinic unsaturation in that portion of the chain exclusive of the 1-alkenyl group. Phosphatidalkyl ethanolamine was isolated from EP preparation and, after direct quantification, shown to account for 7% of the phosphorus of the fraction.