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Preparation and properties of bacterial chromatophores entrapped in polyacrylamide

✍ Scribed by Ho Seung Yang; Kwan-Hang Leung; Michael C. Archer

Publisher: John Wiley and Sons
Year: 1976
Tongue: English
Weight: 338 KB
Volume: 18
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260181009

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The immobilization of Rhodospirillum rubrum chromatophores was successfully performed by entrapping them in polyacrylamide. Their photophosphorylating activity was about 40% of native chromatophores. The temperature and pH optima for immobilized chromatophores were similar to the native photosynthetic apparatus and kinetic parameters showed that the rate of photophosphorylation in polyacrylamide particles was diffusion controlled. Light penetration of the gel particles was not a limiting parameter. Immobilization considerably increased the stability of the chromatophores towards denaturation.

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