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Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolithic Column

✍ Scribed by Li-Yuan ZHANG; Hui WANG; Zhen LIANG; Yi-Chu SHAN; Li-Hua ZHANG; Yu-Kui ZHANG

Book ID
104452632
Publisher
Elsevier Science
Year
2010
Tongue
Chinese
Weight
316 KB
Volume
38
Category
Article
ISSN
1872-2040

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Facile preparation of monolithic immobil
Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides
✍ Liyuan Zhang; Hui Wang; Zhen Liang; Kaiguang Yang; Lihua Zhang; Yukui Zhang πŸ“‚ Article πŸ“… 2011 πŸ› John Wiley and Sons 🌐 English βš– 289 KB

## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the in‐depth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig