Monolithic columns with immobilized monomeric avidin: preparation and application for affinity chromatography
✍ Scribed by Jens Sproß; Andrea Sinz
- Book ID
- 105895943
- Publisher
- Springer
- Year
- 2012
- Tongue
- English
- Weight
- 768 KB
- Volume
- 402
- Category
- Article
- ISSN
- 1618-2650
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## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the in‐depth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig
## Abstract The avidin/biotin system was applied as a general mediator in the adsorption/desorption or immobilization of biologically active macromolecules to solid supports. In this context, model biotinylated proteins (lectins and antibodies) were attached to avidin‐coupled Sepharose. As examples