Preparation and evaluation of non-bonded hyperbranched polymer-coated columns for capillary electrophoresis
β Scribed by Chong-Qi Shou; Chang-Li Zhou; Chun-Bin Zhao; Zhi-Liang Zhang; Guan-Bin Li; Li-Ren Chen
- Book ID
- 116900489
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 119 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0039-9140
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## Abstract A simple method for the preparation of polymerβcoated fused silica capillary columns for electrophoresis is presented. It is shown that the static coating technique used in GC and SFC can be conveniently utilized for the preparation of highly efficient capillary electrophoresis columns.
A new polymer, polymethacryloxypropylhydrosiloxane PMAHS , has been developed and used as both a deactivating layer and an intermediate layer for stable coating of an uncharged polymer on fused silica capillaries in capillary electrophoresis. The deactivation procedure is based on a siliconhydride d