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Preparation and evaluation of ammonia-treated collagen/chitosan matrices for liver tissue engineering

✍ Scribed by Xiaohong Wang; Yongnian Yan; Zhuo Xiong; Feng Lin; Rendong Wu; Renji Zhang; Qingping Lu

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
486 KB
Volume
75B
Category
Article
ISSN
1552-4973

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✦ Synopsis

Abstract

To further enhance the properties of existing collagen/chitosan scaffolds for liver tissue engineering, a very simple method was developed to form noncovalently linked mimic of the liver extracellular matrices. Collagen/chitosan mixtures in various proportions (i.e., 1:0, 3:2, 1:1, 2:3, and 0:1 v/v) were lyophilized or evaporated to form sponges or flat films before they were gelled using an aqueous 25% ammonia solution. The porosities of the obtained sponges were above 90% with various pore sizes. The highest mechanical strength (1.9 ± 0.7 MPa) and the lowest degradation time (65 ± 1.7 days) were achieved by the collagen/chitosan (1:1) matrices. Hepatocytes cultured on the collagen/chitosan (1:1) matrices exhibited relatively high glutamate‐oxaloacetate transaminase and glucose secretion functions 25 days postseeding. Nuclues of the hepatocytes were more elongated and arranged in certain directions on the 1:1 matrices. The cytocompatibility and enhanced biostability of our new ammonia‐treated collagen/chitosan matrices suggest that they could be used as scaffolds for liver tissue engineering. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005

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